m7G(5')pppN diphosphatase
| m7G(5')pppN diphosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.6.1.59 | ||||||||
| CAS no. | 82599-75-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a m7G(5')pppN diphosphatase or m7G(5')pppN pyrophosphatase (EC 3.6.1.59) is an enzyme that catalyzes the chemical reaction
- 7-methylguanosine 5'-triphospho-5'-polyribonucleotide + H2O 7-methylguanosine 5'-phosphate + 5'-diphospho-polyribonucleotide
Thus, the two substrates of this enzyme are 7-methylguanosine 5'-triphospho-5'-polynucleotide and H2O, whereas its two products are 7-methylguanosine 5'-phosphate and 5'-diphospho-polyribonucleotide.[1][2][3]
This is the enzyme involved in the processing of amphetamines of the cathinone group, including mephedrone and khat.
Nomenclature
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'-phosphohydrolase.
Another name in common use is decapase because this enzyme removes the N7-methylguanosine 5-phosphate cap from an mRNA. The process of mRNA decapping controls eukaryotic mRNA degradation.[4]
See also
References
- ^ Nuss DL, Furuichi Y (May 1977). "Characterization of the m7G(5')pppN-pyrophosphatase activity from HeLa cells". The Journal of Biological Chemistry. 252 (9): 2815–2821. doi:10.1016/S0021-9258(17)40435-2. PMID 16003.
- ^ Lavers GC (December 1977). "Cleavage of pm7G from mRNA 5' terminal cap structures by pyrophosphatase activity in embryonic chick lens cells". Molecular Biology Reports. 3 (6): 413–420. doi:10.1007/BF00808382. PMID 593271.
- ^ Nuss DL, Altschuler RE, Peterson AJ (June 1982). "Purification and characterization of the m7G(5')pppN-pyrophosphatase from human placenta". The Journal of Biological Chemistry. 257 (11): 6224–6230. doi:10.1016/S0021-9258(20)65128-6. PMID 6122684.
- ^ Furuichi Y (2015). "Discovery of m(7)G-cap in eukaryotic mRNAs". Proceedings of the Japan Academy. Series B, Physical and Biological Sciences. 91 (8): 394–409. doi:10.2183/pjab.91.394. PMC 4729855. PMID 26460318.