Eaton's agar
Eaton's agar is a type of agar media used to grow Mycoplasma pneumoniae, which was originally called "Eaton's agent" after its isolation by Monroe Eaton in 1944.[1][2]
One recipe for the cultivation of M. pneumoniae (Eaton's agar) includes (v/v):[3]
- 70% Difco PPLO (pleuropneumonia-like organism) agar or broth base
- 20% unheated horse serum
- 10% fresh aqueous extract of baker's yeast
- 1000 units/ml Penicillin G
The original recipe by Chanock, Hayflick, and Barile included 500 units/mL penicillin.[4]
References
- ^ Eaton MD, Meiklejohn G, van Herick W (June 1944). "Studies on the etiology of primary atypical pneumonia: A filterable agent transmissible to cotton rats, hamsters, and chick embryos". The Journal of Experimental Medicine. 79 (6): 649–668. doi:10.1084/jem.79.6.649. PMC 2135382. PMID 19871393.
- ^ Marmion, B. P. (1 March 1990). "Eaton Agent — Science and Scientific Acceptance: A Historical Commentary". Clinical Infectious Diseases. 12 (2): 338–353. doi:10.1093/clinids/12.2.338.
- ^ Dajani AS, Clyde WA, Denny FW (1965). "Experimental infection with Mycoplasma pneumoniae (Eaton's agent)". The Journal of Experimental Medicine. 121 (6): 1071–1086. doi:10.1084/jem.121.6.1071. PMC 2138014. PMID 14319403.
- ^ CHANOCK, RM; HAYFLICK, L; BARILE, MF (15 January 1962). "Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO". Proceedings of the National Academy of Sciences of the United States of America. 48 (1): 41–9. doi:10.1073/pnas.48.1.41. PMC 285494. PMID 13878126.