3-Ketosteroid reductase

3-keto-steroid reductase
Identifiers
EC no.1.1.1.270
CAS no.42616-29-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

In enzymology, 3-keto-steroid reductase (EC 1.1.1.270) is an enzyme that catalyzes the reduction of keto sterols to the corresponding 3β-hydroxysteroids. For example:[1]

 
 
 
H+
 
H+
 
4α-methyllathosterone
 

In this example, the two substrates of the enzyme are lophenol and oxidised nicotinamide adenine dinucleotide phosphate (NADP+). Its products are 4α-methyllathosterone, reduced NADPH, and a proton.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3beta-hydroxy-steroid:NADP+ 3-oxidoreductase. This enzyme is also called 3-KSR. This enzyme participates in biosynthesis of steroids.[2][3]

References

  1. ^ Enzyme 1.1.1.270 at KEGG Pathway Database.
  2. ^ Billheimer JT, Alcorn M, Gaylor JL (1981). "Solubilization and partial purification of a microsomal 3-ketosteroid reductase of cholesterol biosynthesis". Arch. Biochem. Biophys. 211 (1): 430–8. doi:10.1016/0003-9861(81)90474-4. PMID 6946726.
  3. ^ Swindell AC, Gaylor JL (1968). "Investigation of the component reactions of oxidative sterol demethylation. Formation and metabolism of 3-ketosteroid intermediates". J. Biol. Chem. 243 (21): 5546–55. doi:10.1016/S0021-9258(18)91903-4. PMID 4387005.